We're Moving to Revvity.com
Visitors from the United States and Canada can now explore, discover, and buy our products and services at Revvity.com. You will be automatically redirected to Revvity.com
Not located in the United States or Canada?
For lab managers, every day brings new challenges -and new decisions to make. Some are routine, like maintaining staffing levels and keeping consumables shelves stocked. Others have longer-term consequences -and can have a huge impact on the lab’s ability to deliver expected results for all the analytes that are being testing.
Choosing the right HPLC detector for your workflow falls in the second category. Preparing to update, implement or adopt a new HPLC method requires to understand the different hardware and software components that work together to create a seamless analytical experience. The role of the detectors is to determine the presence and concentration of eluting compounds in the mobile phase, and for detectors like mass spectrometers, compound identity may also be determined. Detector types are based on which chemical property is being measured. Broadly, HPLC detectors are categorized into two types: solute-property (or specific) detectors and bulk-property detectors.
Solute-property detectors respond to a particular physical or chemical property of an analyte, and their response is not dependent on the composition of the mobile phase. Bulk-property detectors, by contrast, are the most universal detectors for HPLC, measuring properties common to all analytes by evaluating the differences in the mobile phase with and without the sample. The universal nature of bulk-property detectors places an increased emphasis on the selectivity of the chromatographic column. However, these detectors are limited in their sensitivity.1
Here are some of the most important factors to consider when choosing a detector for HPLC and UHPLC analyses:
Bottom line, the choice of detector depends on the nature of the analyte, the required sensitivity, and the analytical goal. Download this guide to get a comprehensive explanation of the common types of detectors used in HPLC.
Discover the detectors available for the LC 300™ HPLC and UHPLC Systems and learn how our liquid chromatography portfolio can help your lab with HPLC analysis.
1. Michael Swartz (2010) HPLC DETECTORS: A BRIEF REVIEW, Journal of Liquid Chromatography & Related Technologies, 33:9-12, 1130-1150, DOI: 10.1080/10826076.2010.484356
Stay up-to-date with the latest insights and trends, customized to your market and interest.